Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Turn off quantification flag #1089

Open
adamrtalbot opened this issue Oct 10, 2023 · 0 comments
Open

Turn off quantification flag #1089

adamrtalbot opened this issue Oct 10, 2023 · 0 comments
Milestone

Comments

@adamrtalbot
Copy link
Contributor

Description of feature

There isn't currently a method of producing a BAM file without performing quantification, i.e. if --skip_alignment false then all samples will go from STAR to Salmon quant. We could add a flag that is similar to --skip_alignment or --skip_pseudoalignment which prevents quantification, e.g. --skip_quantification, producing the relevant BAM files and any quality control but going no further.

The main use case would be people who wish to generate a BAM file with an RNA-aware alignment algorithm but are not performing a quantification experiment.

The other use case would be people who wish to use Salmon for read-mapping based quantification but also want to produce a BAM on the side:

flowchart TD
    A[Reads] -->|Filtering & QC| B(Filtered reads)
    B --> |STAR| C[BAM]
    B --> |SALMON| D[Counts]
    C --> |QC| F[Validation]
    D --> |Analysis| E[Interpretation]
Loading
@drpatelh drpatelh modified the milestones: 3.12.1, 3.13 Oct 15, 2023
@drpatelh drpatelh modified the milestones: 3.15.0, 3.16.0 May 29, 2024
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Projects
None yet
Development

No branches or pull requests

2 participants