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There isn't currently a method of producing a BAM file without performing quantification, i.e. if --skip_alignment false then all samples will go from STAR to Salmon quant. We could add a flag that is similar to --skip_alignment or --skip_pseudoalignment which prevents quantification, e.g. --skip_quantification, producing the relevant BAM files and any quality control but going no further.
The main use case would be people who wish to generate a BAM file with an RNA-aware alignment algorithm but are not performing a quantification experiment.
The other use case would be people who wish to use Salmon for read-mapping based quantification but also want to produce a BAM on the side:
flowchart TD
A[Reads] -->|Filtering & QC| B(Filtered reads)
B --> |STAR| C[BAM]
B --> |SALMON| D[Counts]
C --> |QC| F[Validation]
D --> |Analysis| E[Interpretation]
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Description of feature
There isn't currently a method of producing a BAM file without performing quantification, i.e. if
--skip_alignment false
then all samples will go from STAR to Salmon quant. We could add a flag that is similar to--skip_alignment
or--skip_pseudoalignment
which prevents quantification, e.g.--skip_quantification
, producing the relevant BAM files and any quality control but going no further.The main use case would be people who wish to generate a BAM file with an RNA-aware alignment algorithm but are not performing a quantification experiment.
The other use case would be people who wish to use Salmon for read-mapping based quantification but also want to produce a BAM on the side:
The text was updated successfully, but these errors were encountered: