minimap2 -I 16G -x map-ont -t 32 -a --secondary=no reference.fasta basecalls/*.fastq | samtools view -bST reference.fasta - > basecalls.bam

samtools view -h -o basecalls.sam basecalls.bam

@SQ	SN:ecoli_SCS110_chromosome	LN:4664852
@SQ	SN:ecoli_SCS110_plasmid1	LN:106875
@SQ	SN:ecoli_SCS110_plasmid2	LN:49252
@SQ	SN:chr1_NA12878_maternal	LN:248952335
@SQ	SN:chr2_NA12878_maternal	LN:242190234
.....
@SQ	SN:yeast_S288C_mitochondrion	LN:85793
@PG	ID:minimap2	PN:minimap2	VN:2.24-r1122	CL:minimap2 -I 16G -x map-ont -t 32 -a --secondary=no reference.fasta basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_0_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_10_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_1_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_11_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_12_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_2_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_3_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_4_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_5_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_6_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_7_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_8_0.fastq basecalls/pass/fastq_runid_2417135a06a11cccf23b4331cfe654a571b445c5_9_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_0_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_10_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_1_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_11_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_12_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_2_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_3_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_4_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_5_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_6_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_7_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_8_0.fastq basecalls/pass/fastq_runid_41d467f0f33918dcca2ef630ecf1b1c3f3fd20de_9_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_0_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_10_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_1_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_11_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_12_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_2_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_3_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_4_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_5_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_6_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_7_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_8_0.fastq basecalls/pass/fastq_runid_5c4249e0f81fb4a3f5aef3558084eb02e076999f_9_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_0_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_10_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_1_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_11_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_12_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_2_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_3_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_4_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_5_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_6_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_7_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_8_0.fastq basecalls/pass/fastq_runid_84fc09cf12b8326e0f77a47d3652d76edb27f593_9_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_0_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_10_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_1_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_11_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_12_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_2_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_3_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_4_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_5_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_6_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_7_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_8_0.fastq basecalls/pass/fastq_runid_8a83948539f27c88b2bd39a499e26cfa553e7ed8_9_0.fastq
cc8739aa-9335-4a77-8681-f13b0b411a80	16	ecoli_SCS110_chromosome	2803510	60	18S80M1I13M2D22M1I6M1D218M1I11M1I47M1D87M3I113M2D10M1D4M1I4M1D127M2D9M1I165M1I50M1D2M1D3M1I4M1I260M1D391M5I59M2I30M4D26M2D7M2D59M1I160M1I2M3D63M2I6M2D141M1I3M1I6M1D38M2I20M1D79M1I27M1I57M3D2M1I16M1D3M1D63M3D46M6D15M1D4M1D49M1D71M1I46M3D67M1D48M2D157M3I28M1I12M1D23M2D54M1D13M2I2M3D35M2D5M1D164M1D146M1I42M2D46M1I6M1D30M3I47M1I11M1I13M1I121M1I73M2I64M1D205M1I564M2I2M2I146M1D38M1D27M1I49M1D33M1I455M1D240M1D137M4D142M1D265M1D137M2D2M2I266M1I103M30S	*	0	0	GCGAAGTTACGTATTGCTTAGATATAAATTGGCGCGGGTTGTTTTTCGCGACGCACGAATTTATCTCATTCAATGGCTGACAAAAATTCGTCACACTCTTTAACCAGAGACACCCTTAATACAGACAAAGAGCACTTTATGAACGCTGCACGCGGGATGTTCTGGCCGATGCTGCTTATTATTTCTGCAAAAATGGCGCATTCACTCTGGCGCTATATCTCCTTTTCTGCGGAATATACGGCGGTTTCTCAACCGGTGAATAAACCGAGCCGTGTGGATGCAAAAACGTTCGATAAAAATGACGTGCAATTAATCAGCCAGCAAAACTGGTTTGGCAAATATCAGCCTGTCGCCGCGCAGGGTAAAACAACTCCGAACCTGTGCCTGTGGCAGAAACGCGTCTTAATGTGGTGCTGCGTGGATCGCCTTTGGTGCCAGACCCGGCGCGGTTATTGAAGAAGGCGGTAAACAGCAGGTCTATTTGCAGGGTGAAACGCTTGGCTCTCGCTACAACGCGGTGATTGAGGAAATCAACCGCGACCATGTGATGCTGCGTTATCAGGGAAAAATAGAGCGTCTGAGTCTTGCAGAAGAGGAACGTTCCACTGTTGCCGTGACCAACAAAAGCCGTCGCGATCGTACAAAGCAAGCTGTTGCTGAGCCTGCTGTCAGTGTGCCAGTTGAGATCCTGGCTGCCGTGCGTCAGGCACTGGCGAAAGATCCGCAGAAAATTTTTAATCATATCCAGCTTACGCCTGTGCGTAAGGAGGATTGTCGCATTATGCAGCGAAGCCGGGGGCAGATCGTTCTCTGTTCGATGCCAGCGGTTTCAAGGAAGGCGATATCGCCATTGCGCTAAATCAGCAGGATTTCACTGATCCACGAGCAATGATTGCTCTGATGCGGCAGTTACCTTCAATGGATTCCATTCAACTTACGGTTTCTACGCAAGGGTGCGCGCCACGACATTTCCGACGCGCTGCGCTACCGACTATCGCCTCGCCTTTACTGGAAGAAGAGCTGAACCAGGATATCGATGAACTGCATTTCGCCCCGTTGAATATCGACCAGCAACTGGCAACCGTAGCAGTGATTCACCAACAGCATATGCGCAACATTGCGCAGTGGTTGCAGGAAAACGGCATCACCCGCGCTACCGTCGCGCCGGACTGGATGTCCATTCCTTGTGGGCTTATGGCTTGCGATGCGCAACGGGTTATCTGCCGCATCGATGAATGCCGGGGATGGAGCGCCGGGCTGGCGCTGCTCCGGTCATGTTCCGCGCCCAGCTCAATGAGCAGGATTTACCGCTTTCGCTAACCGTAGTCGGCATTGCACCGGAAAAGCTGTCAGCATGGGCTGGCGCAGACGCTGAACGCCTGACCGTTACAGCTCTGCCCGCCATTACCACTTATGGCGAACCGGAAGGGAACCTGCTGACAGGGCCGTGGCAGCCTCGCGTCAGCTACCGAAAACAGTGGGCGCGCTGGCGGGTGATGATTCTGCCGATATTGCTGATTCTGGTTGCGCTGGCAGTGGAGCGGGGCGTGACGTTATGGAGCGTCAGCGAACAGGTGGCGCAAAGCCGCACCCAGGCGGAGGAACAGTTCTTAACGTTGTTCCCGGAGCAGAAGCGGATTGTGAATTTACGCTCTCAGGCAAGGTGACGATGGCGCTGAAAAAATATCGCCCACAGGCCGACGATACCCGGCTGCTCGCCAGGAGTTGTCAGCGATAGCCAGCACCCCGGAACGTCACTTTCCGACATCGAAATGCATTTTTACCTGATCAAAAACGCCAGATACTTCACCTCCAGCTACGGGTCATGAACTTTGCCAGTTTCGCACAAACCGCGTAGTGTACTGGCAACTGATTATGTTGTGCAACAGGACGCGTTACAGAAAGAGGGTGATGCGGTTTCCGGCGGCGTAACGCTGCGGAGGAAATAACATGTTACGCGATAAATTTATTCACTATTTTCAGCAATGGCGTGAACGCCAGTTAATGCGGCGAACACTGGCTGGCACAACACCTGGCGGGGCGTTCTCCGCGTGAAAAGGGCATGTTACTGGTGCTGCGGGTGTTCTTGCTTAGCGTCGGATATTACGTCCTCATCTGGCAGCCGTTGAGCGAACGGATTGAGCAACAGGAGACAATATTGCAGCAGCTGGTGGCGATGAACACCCGATCGAAGAACGCCGCGCCGGATATTATTGCAGCGGCGAAAAATCGCCACAACAACGCCTGCGCAGGTATCGCGGGTCATCAGCATGACAGTGCTTCCGCGCACTGGTGGTCATCAGGCGGATAGCCGATCGTGGGGAGAATATTCAGGTCTGGATAGAACCTGTGGTGTTTAATGACCTGCTGAAAATGGTTAAACGCACTGGATGAAAAATTATGCGCTGCGGGTGACACAAATTGATGTCAGTGCTGCTGAGAAGCCTGGGATGGTGGTCGCAGCGGCTGGAGTTTGACGGGGTAAAGTATCAGGAGATTAATATCAATAAAATTACTCTCGTTAACGTGGCGAGAGTAATTCCAGACTTTATAAGCTAATTTTTTTCGTGAAGGTTATTGTCAGATCCAAAAACCCGCCTTGAAGCGGTTCTTAAGAATTAGTGGTGCCCGGACTCGGAATCGAACCAAGGACACGGGATTTTCAATCCCCTGCTCTACCGACTGAGCTATCCGGGCAACGGGGCGCATTAAACCTGATTCGCCTCAGCCCCGTCAATCAAATTTCTTCAATTTACTGCAGACTGCACAACGTATCACTTTGCCGATATTGCACGCATTGTCAGGCAAACCAGGCGGTCCAGGCGGCGGAAAGCGGCATTACAGAAGGAGTGCTGGAAGCATGTTGACCACCGGGAACATTTTAACGCCAGATGCGTAATCCGGTTGCCAGCAGCAATAAACCGCCTACGGCGCTGAAGTCTGCCATCATCGACGGTGTGGTCAGCGGTAAAATCAGCGCGGCAGCCCACGCCAGCGTTAACTGGATGATCAGTAATGGGATACTAATCACCGATACCGCAATACCTTCCAGCGAGCAGGCGAAGATCATCGCCGTAAAAGAAATCAAGAATGACTTGGCGATTAAAATACTGATCGCCGGTCATCCCTTCGTTCATCGCCCCGAAGATCCCGGTGCCGCTGGCGCAAACAGGACAATACCATGACATACTCCTGAATAAAAGATTCATGTGCTGCCTTTACGGAGTGACGAAACAGATTTTGTGCTTTGGCGACTGCTGTATTACCACCTTTCTCCAGCAAACAAATTTCGCCAATTAGAGCGCCAAGTAGCGTAGTTAAAACTATCGCTGGAAGGTTGGCGCATTTCACCACCAGTAAAATACCAATCCCCAGCGATGCCAGACCAAAATTGATGTCATGGAGACGCGGATACGTTCCGGTAAGCGTTGGCTGAGCAGTGCGCCGAGAACGCCACCCAGTAAGACTGCACTAGCGTTGATAAAAGGGCCGATGACCACAAGAGTTCCTGTTAGCTGTATTGTTTGATTTTCTTTATTATGACCGTTTGGTGCCGTCGATGGCTTGTTTGTTCGCAGTCCTGGCTTGCACGCTTTAGCGAAAGGTGCCATGATTGCGCGAATTTTTCTCTCCCTGTACGGAGTTTGCCCGATGCACGCTATCATCTCCTTACATTCTCTCGCTTATCGCCGTTTCGCGCGAAACGTTTCCCCTTTCTATGCTTACTGCTCATGCGGGTGAAGTTAACGCACGCTCACTGCAGGACAACAGTAAAATCAGAGCGTTTCTGCTTTTACTGATGTCTGGCGGTCGGAGCTGGTGACCAGTTTGACCCATATCTCATGGGGCAGGGTTTTCCCACCTTGTCCGGTATTCTTACTTCCCTGAAACGGGTTTGCGCTTATGAAATCAATGAATATTGCCGCCAGTAGTGTGAACTGGTATCCCGACTTTCTTCTCATCGTCGCGTGGTGGCGTTGGGAGATACTGATTTTACGACGTCGCGGCAGTCGTCATTACCGCTGCGGATAGTCGCAGTGGCATTCTTGCGTTGCTTAAGCGCACCGGTTTTCATCTACCGGTGTTTTTGTATTCCGAACATGCTGTTGAATTACCTGCGGGCGTTACGGCGGTAATCAACGGCAACGAGCAGCAGTGGCTGGAGCTGGAATCCGCAGCCTGTCAGTATGAAGAGAATTTGCTTTTCACCGTTTTATGACACGCTGACGCAGTACGTTGAGATGGGCAACAGCACCTTTGCTTGCCCTGGACATCAACATGGTGCGTTTTTTAAAAAGTATCCTGCCGGACGCCATTTTTACGATTTCTTTGGTGAGAACGCTTTTCGCGCCGATATGTGTAACGCTGACGTAAAATTGGGCGATCTGCTTATTCATGAAGGATCGGCGAAAGATGCGCAGAAATTCGCAGCCAAAGTCTTTCATGCCGATAAAACCTATTTTGTGCTGAACGGCACATCGGCAGCGAATAAAGTGGTGACGAATGCGCTGTTAACGCGTGGCGATCTGGTGCTCTTCGACCGTAACAACCATAAGTCGAATCATCACGGCGCGCTGATTCAGGCGGGGGCGACGCCGGTCTATCTGGAAGCTTCACGCAACCCGTTTGGTTTCATTGGCGGTATTGATGCGCACTGTTTTAATGAAGAGTATCTGCGCCAGCAAATTCGCGACGTTGCGCCAGAAAAAGCCGACCTGCCGCGCCCGTATCGCCTGGCGATTATTCAGCTGGGAACCTATGACGGCATTCTTCGTCTATAACGCCTGTCAGGTGATCGATACCGTTGGGCATCTGTGTGATTACATTCTGTTTGATTCCGCGTGGGTCGGTTATGAACAATTTATCCCGATGATGGCGGATAGCTCGCCGCTGCTGTTAGAACTTAACGAAAACGATCCGGGATCTTTGTGACTCAGTCGGTGCACAAACAGCAGGCGGATTCTCACAGACGTCGCAGATCCATAAAAAAAGATAACCATATCCGCGGACAGGCGCGTTTTTGCCCGCATAAGCGTTGAATAACGCCTTTATGCTCCATGCTTCTACTCAGCCCTTTCTATCCGCTGTTTGCTGCACTGGATGTTAACGCCAAAATTCATGAAGGGGAGAGTGGGCGTCGGCTGTGGGCTGAGTGTGTTGAGATAGGGATTGAAGCGCGCAAGGCTATTCTTGCGCGCTGTAAGCTGTTCCGCCCGTTTATCCCGCCCGTTGTTGATGGCAAATTGTGGCAGGATTATCCGACATCAGTGTTAGCCAGCGACCGCCGTTTTTTCAGTTTTGAGCCGGGGGCGAAGTGGCACGGCTTTGAAGGATATGCCGCGGATCAGTATTTTGTTGATCCGTGCAAGCTGTTACTCACTACACCAGGTATCGATGCCGAAACCGGCGAATATAGCGACTTTGGCGTTCCGGCGACGATTCTGGCGCACTATCTGCGTGAGAACGGCATTGTGCCGGAGAAGTGCGATCTCAACTCCATTCTGTTTTTATTAACTCCGGCGGAAGCCACGAGAAGCTGGCACAACTGGTGGCGATGCTGGCGCAATTTGAACAGCATATTGAGGATGACTCGCCGCTGGTTGAGGTGTTGCCGAGCGTTTATAACAAGTATCCGGTGCGCTATCGCGACTACACCCTGCGCCAGTTGTGTCAGGAGATGCACGATCTGTATGTCAGTTTCGACGTCAAAGACCTACAAAAAGCGATGTTCCGCCAGCAGAGTTTCCCGTCAGTGGTGATGAACCCCAGGATGCGCATAGCGCTTATATTCGCGGTGACGTGGAGTTGGTGTGGATTCGTGATGCCGAAGGGCGAATTGCGGCAGAAGGGGCGTTGCCTTATCCACCTGGCGTGCTTTGCGTGGTACCCGGGGAAGTCTGGGGGCGGTTCAACGTTATTTCCTTGCACTGGAAGAAGGGGTGAATTTGTTGCCGGGATTTTCGCCGGAGCTGCAAGGTGTTTATAGCGAAACCGATGCGGATGGCGTGAAACGGTTGTACGGTTATGTGTTGAAGTAAGAATAAAAAAACGGGTCACCTTCTGGCGACCCGTTTTTCTTTGCGTAATTAGTGGCTAACCGTCTGTGTGCCTGTCGGGACACGAACGTGTTTATATTTGAACATCGCCATGAACGCGAAGGCCAGAACCACGGAGTAACCAGCGAAAAGTAACCATACGGTCTGCCAGTCGGTAATGCCGTTTTGGGTGTACATCTCAACAACTTTACCGCTCACGATGCCGCCGAGGATACAGCCGAAGCCGTTAGTCATCATCAGGAACATCCCTTTGCACTGGCGCGAATTGCCGGGCTAACTTCTTTTTCGACAAACACCGAACCAGAGATGTTGAAGAAGTCGAATGCGCAACCGTAAACGATCATCGACAGTACCAGCAGTACAGTACCGAACGGAGTCGGGTCGCCGTGCTAAAACAGCGCAAAACGCAGGATCCACGCCACAATACTGATCATCATTACGTCTTTAATACCGTAGCGGCTTAAGAAGAACGGGATGGTCAGAATGAACAGGGTTTCAGAGATCTGCGAAATCGACATGATGATTGACGCATGCTGCACAATAAAGCTGCTGGCAAACATCGGATCTTTGTCGAAGCTGTGCAGGAAGGTATTACCGAACATGTTGGTAATCTGCAGTTCCGCGCCCAGCACTGATGAATCCCCTAATGATTTTTATCAAAAATCATTAAGTTGTTGCAGATACACATCTTGTCATATGATCAAATGGTTTCGCCAAAAATCAATAATCAGACAACAAAATGTGCGAACTCGATATTTTACAAGCAATACGTAACTGAACGAAGCAATACTT	&(*-58==<<<;;:;===66=6+*+++*+/19:>=666588=<;4.-..88:;;<:9978?;;<<?=@<9/.++*+**8875:54,'%%%'*1++++*(4E>@?@?>;;0////54566A@@@>ACE>=:6&&&)''''(&&%%''1888678=?>=<::961-,,,;>(''''(<=>FC@=7446>ACEE@?@??@A=<965/+*+AB@@>>EC@:{993))*222487=<<8{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{E{>>=<>556:=GGMGAAA@EBDEF@=<>>?CC?>><;;;<>>?>@DECC::::?>??@BCBA@A@?@?@@@???=>=><=>;;983241/'&%&.5=B?@===A@=<<5555>>BC<<<<?>B<57==<<?@AAD:;8*)))()))HHCA><<=?@@?56<<BAA@=;<=><;;;;>>?CBBB>>>>F><8999=>==:,7,*))+()(446<?><:+*,,&(()*9:<<<;6.*{((-59000****.:95119E@AA541)()())32368899;:=<<;<@>==<>>?97:>99.+('(*245:996.0007340*&'-46445@A@?=89==<870,,,234559<<<=>>AA<;6556:;=DBB?=>@==:67A733767.--,/,,9978;0///34::91..''(?@CA=;;9998;{FC=A=<==><{{B865021+(8::<1*)'('''../CDAB=<<<BCBB>?>?;;;;;>==?=<==?=<9;;?=>86668&$$$&&'8?@???>?@CABB@?96)()77;:98:::::2118=;<9:=::;;:/-**--*,7>>@??@@A::4/1>;;8:A;9817624567AA<9787/..8())(''&'(*..>>@@<2<6677ADJ{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{{,++(4BA@::A=/87('&$''&(+++.('%&%&).::9<;;<<=>IA@9+:>@C?>><==<==91**(&&$%&$$%&))0-.1{5'%&&'112>?C@?9<8988>B>=7777>7632389;??>??<?>??>?@@=::A<<<B,++(&&&',+)*02656==@@<=5567EBB553244C<;::41//@DDDDE>-++7788788:?=<<<=?FC?@??>?@D7655556540,,%%%%%%%%$$&,/=--,%$$$%%))48555-**A@><++90////.,,*,69@F=::CCBA@6664111122{DODA?={?=>?ABB??>?>>=:/->?00/+)*;@;<=:3<;998/..-.-3@BF:444@BB>=>=@?<88;<./870+*('*.**)))'&&%''(**3:9625887;A;97/-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c08f78a3-3ea1-42a4-ad47-10964168365b	16	chr5_NA12878_paternal	90633110	55	16S117M3D81M2D118M1D235M3D42M2I141M1D56M1D35M1D2M4I280M1I353M1I21M1D203M1D28M1I283M1D22M3I128M1D197M1D28M1I35M1I46M1D16M1D42M2D117M1I248M1I122M1I70M1I22M1I175M2D295M1I14M1D13M2D2M4D282M2I210M2D26M1I44M1D221M1I235M2D106M3I4M2D55M1I93M2D43M2D61M1I9M1D76M2D75M1D230M1I129M1D78M1D11M1D69M2D36M1I133M1I134M1D55M1I58M1D71M1D70M1D55M1I49M1D6M1I46M28S	*	0	0	TCAGTTACGTATTGCTTGAGAAACCAGCAATATGTAAAATGTATAACTGCAGGGACACTATATGTTATACATTGCAGTTCTTTAAATGTAAAGATGCATGAAGTTATAAATGGAGTTCTGAGTAGTCTTCTGATGGATTTTGTCTGGAGGAGAAGGGAAATGGGGGATGATTGGAAGTGTGGGTCAGTGGAAGACAGGAAGGAGATGTGGATGACTCTGATATTTGGCTTTAAGGTCAAGGTAGTTGGATGTAGGTGGCAAGGAGGAAAAGCAGGTGGTGGCACTGCTGATGATACTAGTGAAGGGGCATGGAACAGGGACAGTGATTTCTGTTTGGATATTGTATATGTAAGGTGTTTGTGTGACATTTATATGATGTCTGTGGCAGTTAGTTATAATGATTTGGAATTAGAGGGAGAGATGCAGAGATTATTAAGGAGCAGGCATAAAATTATAAGATAAAGGGTTTAGGCAGACCCTTGGGGAATAATAACAATTGAGGGGCAGAAAGAGCTCCAGAGGCCAGGGAAGGAGATTGAAAAGGGATGGTGAATGAGAGAGGAGGAGAACATTGCGAAGTTATTGGTGACCTCTACCTACGCTTACTTTAAATGCTCCCATGCTGTTAAGCTTTTTTAAAAAATTTATATTCTATCAATTCTTACTACTTTTTGTGTATTTGTTTATTATAGTTGGAAACTGTGGAGTTGTTAAACATAATTCCTCTAATCCCACCCATAAGCCCTAGATTTGGGAAATCTGCAATATTTCTTTACTGGTTACTCCAGCCATTGCAAATGGAGAAATTACTTTCTCAGCAATCTTCCAATTATTTTGCATGAACATTGTGAAGATTTTGCTGCTGAAGTGGTAAGTAGGCTCTTTCTTACTGATGGGGGCTAATGAGTATGAAGTAATGTACAGACACTATTTTTAAAATAAGATCAGCATATAGGGTTAACATCTTAAAAAGCTTCAGCATGTACAAGAAGCCTTCAGTATGTCATTTGAAATAGGTAACTGATGAGTATGTGATTTTTCCATCATCGTTTACAGTATTAGATAGAATGAAATTGGAATTAATTGCTCACAGTGAGTGTCGGATATTTTGATGTGACAGCTTTCAGATAAGTTCTATGACATTCGATACTACAGTGGTAGCCAAACATAAACTTTTTTTTTTTTTCTTTTGAGAGAGGATTTTACTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCAGCTCAGTGCAACCTCTGCCCCGGGCTCAATAGATCCTCCCACCTCAGCCTTATGAGTAGCTGGGACTACAGGTCTGTGCCACCACTCCTGCCTATTTTTTCTTTGTATTTTTAGTAAAGATGGGGTTTTGTCATGTTGTCCAGTTTGGTCCCCAACTACTGAGCTCAAGTAGTCCACCTCCCTTGGCCTTCCAAAGTAATGGGATTACAGGCATGAGGCATTGTGCCTGGCCTATAAACCTTTTTTTTTTTTTTTTCTTAATGCCAAGACATGGTACCACAGGTGTGCTTGACCTACTTCATTTCTGAGCATTCCATATGCTTGGGTTGGAGTGAGGTGGCCAGGTTGGGCCTTATGCTTGATGATGGAGATGATAGTGGTAAAGTTGTGGAATGCTAAATCTTAAGAATTTTTTACTTTATATATACAATATATGTAAATTTTTGCATATAGACATACAAAACGTCACATGCACAGCACCCCATCCCTCCCCTTTGCCCCCACCCCGACATGCCCTGGTTGAATTTTTCTCTTAGGGATGGCTCTTCCTTCAGCCTTACCATTTCCTGAGTCATAGTTGATCCCTGAGACCTTCTCCAAGTCCCCCACACTTAAATTTGATTTAAACCTAGTTGAGGTTTGGATCAGAAAGAAGGAGAGCTGTTTTTGTTATCAAAGCCTTATGTTAAAATATGGTGACCTCAATTTCCATTGTATATTAGCTTCTATAAATGGACAGAGAAGTTTCATTTTTCAAGATGGTTTTGATTTTTCATATTTTCTGAATACACTCTTTTCTTTCTCTTAAAACGGAAAAGCATTTAGTTTGCTCTTAAAGAGTTGATTGCTTTTATCTTAGATAGCTGTAAATTTACTGTTTCATATTTTTCCCTTAAGAAAAATTAGCTTTTAGGAAGTTTTGGGCTCCTTGACCTGCATTCTTCCCTGTTGGGAAACAGCAGAAAAACTAGGAGTTTTCCTTTGTTTGTTCCAGATACAGCCTGGATGATGCCCACTCCCTCAAGGTGATTCAGGGCCCCAGCCCTCTGTTATAAGAATCTTTGCTCACTAGTCTCAGGTTATGACGATTGTTAGCTGAAGTAGCAGAATGATTAATGGAGAAAATGCCTGTTTTTTTTTTTCCAAGATGAGCTTAAAAGGGCAAAGAAAAAATAAAACAGGATACTCCTCTTTTTAGGAGCATAAAAAGAATAGTGTAATCTAGCATTAAATATTCCTTATATTCCTCCTTTGAAAAAACTTCATCATGATATGCAGCGATCCTTTTATTATACATTTTAAAGTGTACTGCCCTTGAATGAAACCTCCAAATACAATTTGTTTCAGAATTATTATAATAGCTTCCATTTATTTAGCTTATACTGTGGAGAGGCACTGTCCTAGATACTTTACTTTAAACAATTCCTAATCCTCACCACAAACTTGCCAAATAGGGAATCATTAGCTTTATTTCACATGAGAAAATTAGGATCAGAGAGGTTAAGTAACTTGCTCAAGGTAACCCAGAGTCATGATAAAAATATCTGATTTTATTTCTGTGGTATTTATCCTATTGGTTAGAATTTTTTTTTTAATTTTACCCCCATCACTTAGGATGTATTTTATATGAGATAATTTTCCAGTTTTAATCAATCGAGTTTATTTTTTCAGTTTATATTGTGATAGTAAGTTCATTTTATTTTCAGAGAACCTCTAATGGAAATATATTCTTTAGCAAGATTGCTGTATGTTTTTCACAATTCTTTGGGTTTTAAAATATTTGCCTTTTTATTAAGGCAAACCCAGATTAGACACATTTTTGGGTGAGGGTAGTTTTGTATATAAATTTGATTAGCAAAAAATTAATAAAAATATATCTAAAAGAGATTTCCATGCTTAAAGGATTGTTTGGCTGTGTTTTAATGCATTTAATAGTTCGCTTCTCTATATAGAATACATATGTTCACACAGTAATATATGTACAAACTAATATCAATACCAAAGCTTATATAATTTCTGAACATATATTTTAGAAGAAATTGTTCTGTTCTTTTCTTTATAAGGTATACATTCCCTTACATCGGGATGGAACTGATGGCCAGGCTACTGTCTACTGGAGTTTGAAGCCCTCTGGCTTTAATTCAAAAGCAGTGACCCCGGATGATATAGGCCCCTTTAATGGCTCTGTTTTGTTTTTATCTGGGCAAAGTGACACAACAATCAACATTACTATCAAAGGTGATGACATACCGGAAATGAATGAAACTGTAACACTTTCTCTAGACAGGTAATAACCCATTTAGGTAATGTTTAGTATCATTTATGTTTGAGTTCTCTTCAATAACTTTGATTTTTTTTTTTACTCTTTTTTTCTATATAGCAAAAAAGTCAAGTAAATAGTGTTATACTGGCCTTCAGACTTTTCTGTTATTTGTTGATGGCTTCTGATTAGACTATGAGTATAATTATATCTTTCAGTGAATTTTTACCCTGCTTTTCATTGTGAAGAAGCACTACAGAGAGAATTTATGGAAGGTATCAGATAAATACGAAACATAGCTAATATGTTGTTATATTAAAAAAAAAATAGGCACCAGCAAATCTAAAATGTTAGGTTAAGCAAACAGTTCTGCTGGCTTTATGTTCAACAGAAGAGAGGACTAGAGAGCTTGTGAGTAAAAAGAAAACATTAATCTTCATGTAATAAAATTCTCTTTTTTACATGGAACTTAAACATTAATTTTATGATTATTTTCTAAAGAATCAAAAATTGCACAAAATTAAGAGATACTTTTTCAGTAATAAATAATAAGAATCAACAGCATTTCTCAGTGGGTAATTTATATTAAGAAAACTTCACAATCTCTCAAAGAGTTTTTTTTCAGTTTTTCAGAAAATCCTTTTCTAAATATATAAAATACTGTGTTTAATGATTTAATAATTCTAAAAATCAGAATCAGTCTTATCCCTAACACAAGTCTCTTTATATGAAAGTTGATTCATATTAGAGAAGGTTACTGTGACTTGAGGGCTTTGTGCAATTCATATTTAGAAACAATAAAATTCAAGATAAATTCAAGGGACATTTTGTGTTACATTATTCAGGACTAAAGTAGTAAGTTATTTTATATAGTCCATGGATCTCTCTATAACAGTGTCTTATAGATTTTTTCTTTGTAGATGCTTTGTCTAAACACATTTTTAAAAATCTGATGGGTAACTGATTTTTATTTTTATGTGAAAGAAAAGTATAGAATGTAAGCAACAAATTATTGAATGTCCACTGTTCCTTTTGCTTTCTTTCAAAACCACGTTGTGTTTACTTCCTTTCTTACATAAAGAGTAAAACAAAACAACCAAAATACCAAGCAAACAAAAAAACCAAAAAGCAAAAACCAATTTAACACACACACACACACACACACACACGCTCGCGCACACACACACACAGAGTCTAGATGACTTATGGTTTTAATATATGTTTCAGACTCGATCTTAAGAAAAGGTAGAGCTTCACGATGAAAAAATCAGTAAAATGTAATGAAATGTAAATTGGAAATTATTTTTTTCTATTTTTGTTTTTTTCCTTAAGTAAGGTGTAAGCATTCAGTGGACAATCTTGAAAACAACTAAAGAAACAGAAACAGTGATGATAAACCAATGCTGGGAATCTGTAGAAACTTTGTTAGCTTAGCTGACAGTTTCTACTCAACTAAATGTTTAAAGAATGATTTACACTATCCATCTGCAAATTGATAGTGACTATTCTTTTATATTCTTCTAACTATTCATTTTTAAATCTTATGTAAACATTTGAAATTAAGTATAATTTTTAAGGGTTTATTTTGTTCTTCTTTCTATCCCCTATGGGTAAAAATAGTACATAGTAAAAAAAGTACATAAGGAAGTACAAATCATGAGGTAGAAAACTCAGAAATTATTGGTTCTTTTCAAATAGAGGAAATTAGTTCAAATATATATTAAAATGTTTTAATTTGACTAGTAATTGGTGCATACTTGTATTATCTCATTTGTGTGTGAACAAGAATGAGCAAATTACATTTGAGTGTTCAGAAACATTTAGTTAAATAAAATTTAGATACTGTATTTTATCAAAAACTCCCTCTTAATTTTGCAAATTTTTAACAAAACTTGAGTGAAAAGAATAAATGAATGATAGCAAGCATGATTACAATAAATCACATCACAATATTGCAATGAAAATAGTTAACAGTTTATTGTGATAACAGTTTATAATTTCCTCTCAATGTCAAATCCAAGTTCAAATTTCTATATTTCAACTCAATATAGACAAAAGTTGCCCTGATACTAAAAAACTTGTGGTGCTTTCAAATAACATGCTGGTTAAAATATTCTATTAGTTTTCCTCAAATCACAAACAATGTCATTATGCCAGTTTATTTCAGAAAAATTTTGGTGTATGTGGTATAACCCTATATTTATAAATGGTGCATTTGACTTTAAAAAATGAAGCTCTAGGGCTCTACATTTGACATGTGCTTTCAAGGCTGTTTGAAAATGAAATACACTGTGGATTTGGAAAAGTGTTTTGGTTTCCTGATGCCAAGTTGTATTTAAGTAATTTTTTACAGAATTAAAAAGGTGCAATGATATGGCTTCTTCAGTATGTTCCTGTGCAAATGATATAGGTATAGATTTCTCTAGTTACACAGTTTTGTTTTTTCTAACTCAAAGAACAACTGGCTTTAGATCTGAGACTAAAGGTTTTTATTTTTTTTAAATCTAATTAAAGCTACTAAGTAATTATAAGCAGGGCTGAATGAAATTTTGTTCAGCTGCTGTGGTTTGTGAGCCTGTACAGTGCTTGGCAGGCACAGGCGCCTTGAGGAGGCCTGGAAAGGAATGTCTTGAATGGGTCTTGACAAGCTTGCTGGCCATGGGGTCATCTTCCATAAAGGGAACTGCCGAACCTCTTGCTAGCATTTGTCAATAGCAAAGAATCCAGGGTGCAGAGTTCCCATAAAAGCACATTACTAGCTCTTGGGGAAAAAGGAAGCCTTTCTTTAGCTTTATTTTTCCCTTTGCCATTCTAAGTTTCTCACAGTATTAGTGAGATTTTAGCAAATGGTGCCTGCCTTCCTTTTTCCCCTTCCCACACTTCTTTCTGTGTTGACAGGGTAGAAGCAATACGTAACTAAACGAAGTACAATT	$$)055569@?====66A?15/.,,222<>>>=?@DC@@@?@@??>>A@<020..001<C=<;:=?C@A@=6666:;::::A?DA?=?@?@A?@>==;==;97=ADBA@<<;<?>=9:>A?>?577886..&$%)5:9:7664103001@=;;::<>;>>?ACA>>>>BA@>@E<>;=<?=>?;9:*),??>>>??>??2=@@A>=<1@B<<=/C44>CCCB?AA=><;===;;:<AA6A?8878<?<;>><;;:;<<><3@>?@B:FCCHA@@>>>???@=;;<@A?BA@>>>>??ABFC8777<@C@?>?A=;9::<?@CAC423-(((()8;;@AB>>==BA::99<==>?A><;A{B??@@AADEJ?=<;<;:;<<<<;<;<>>>==?>>>??<=>=A???BCBB?=?==@E{B@C:>0011768@MFFA<;<<==94445BA:;@A?===@A<>>==999;=<<88446=<>@5=99??>=?@@B<=<9<;;=:=<<??AACB@?>==?>?DB><=:<?=<@@A:=>?>?5:A=@=>====>>>@BBAB{{{{-,-96:76(''(985668@>>>>??@@@?<9:510)('(/30,*(('(&&&&%###&'(()++,,/-,,.004<?A?<;DBC:;>=?EEA;::<@AAB?2444==;9878995>>BABCFB>>??A???@=>>=@??BA?==>=><=><>??GD=<<=@BEC>>>=?>@@@??<=<<=>=<9:789?@ACA76@??A?@@?;:000009,+>??A@>202:;-++-=>=??@BCADCD@A@>>??:>+***0<=9:>??<<;<=>??75555C@GB+**+/+(&$$$$$$%>>???FBCC?;;;9:AC7759A@???=<:9=>,,/+'(=>>?>9?>>::;=??>=<=>>>?>;;;=A??>>==<;<<==?CFNBC?@B?@?=>>@?<<;<@A?>><=<AB@?@=>>?BEFB>=;;@A>==6444<=>>=?><<>;:99:;>><<<>BA@AA@A>==>>@?>=>====<<<==?@BCFEI><<?>=:999=;<<<<>@@8??@@B89A@=@@=::::<?A>=;:9797:==?@>?>?>>64447?A5554++>>=<=>9110///00>=1()//,++/5::<875459?>=====<<<;;<<=>>=<;;;98:;>?BDCABDAA;;3200>89{{{@::;:<;9;43B@@<:::=@><9878?=<>>>D>?>;:6678B??:769?=999:@AA?=@750-,2))*689:>:8779<=CCHD>@?28=DA><=9>??BABBA:@DDC?A>?>>==::;<?@ABD:..@=@;===?>><?FGFC;:=:A??;@ADGAA?:::;=<=<==>AED>===:<>><;;;@>88888<:;:;>9@?=>@?=?;=4570000;<?=;988:=><;<<:?@A?9;<;;5442345>;:EABD?>=>B9:98;:;=>>>>@@BA>>?>=86566?F{HFGGCCD?;0./669<?===??><=<<<<;:97787647>{5566A><==<=;9:;=7842/0<;<<<==?>52**/.---.22239878<9:<5777<<<;=><;<><?=<<==B@@?=>=>9:99467+)),)899=????<;;;?A=<<<=>==-'(A?@>>DDC>@<:9?:A?{{{C::9:<C{C:999=>?@986888<AC;;97323446<952(&*+=;;::<;:;:;:=9:9<>====:=-,(;;;>><;:<4+++9?>=<;=9::=<???<::763{03094,)8;<:;:;<=:9:<==;99:78888;<?>:=::>===><;<<>?:987822=;33)'(2617678:DB=:9==::9;;=>>>@ABD?>@<9::;<<<;<<<==?63388734AD?>>=9=A</.--./03-***+?=ACC;:9:;<<?>?A?A?>>?<===:97769<?4@=<;<=<<<?@?@@>:433))59?=>9999?<?54468799<=:B@52B><;;<<<>JA@?@BDJ=3,,,--544338:><<<<6:96/262.(..{-()6=91001DA@6455??>;;<;;::=411EB>;;==AAB@?@?DBA@???>=<<;<9:;<?A@AA@89*)))8:<=???CEK<:88><@A?AIFLFEBA;;99789767=9>>A:6:;763.33((21455;>=>;::;:8,,,,/.1:<@====@?ADEA?7;312=>@JHD@B?<?;>;:::==<8888;;;<=?<;:=;;;;<<<>>9997<==<===??@AB@:99:<>99:==D??<===A@CC?AC<=<?<<;<<>=><<<>>;>=>>=<<<74336=>>=<<<<<<??><;<<@@21//>?@>>;;::<A@=8&$$$').258=>A@A:9:>@>==><;;;<ABBC=;9>B<5=BIDDA@<<A@>?==>?C@:7554.261AD>502<==>?BCAEB@??=<;<=>BCB@@?>??@@?@BDB=8/30A@E?=2./7665.==BCDE;::;>AB@>>=>??>=<:31125::;64?>@?@?@?@>@>:<@>>;;>>==;88<>?>????@N=<;;;;;?=<==>?BCCA?AEC@?>?CDBCBCACBAC@A75401=C>>;:733667=>=;9:=<??BG==<<<;;;<??<=<<:::A>;791)<>>=<<=<:88:;;9=?B;;::>?B@@A8><<<@B@=:9:;?BB=<<:9999:=@AA<<<><>:9<???D??:50000/5{A=4322777C@@@<A???><=;:;::;;<>=>>???A?>@CGEBD@-,,++.2486;>==<;;;<;<?AA@=<<=>=?>>?@>?:87:<CGHD@@A;;;=<<=87;<:;<=<==<;:45<><=CHCC{DD@?=<<;.++*.;999<<=ABA@?>..--.4/.../7>DFC>=>=>>BABF@??@>?>=?>?:::;>=@BD@??BDDA???>;:76867:;=:9;DA@C{I?@BD{A><<<=<>B666<<==>>??L{EA@>??@=<<?-55143/**,<><<?B>201212;?AC::8999<>?>===>>?==:9=A=BDEEB9998;=;::9;;<<>BA6569{{=;;<::;B?><<:=ADG{D;>?AACCGEFAC{;34+*+((/.2062666>>>>=3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NM:i:111	ms:i:8317	AS:i:12156	nn:i:0	tp:A:P	cm:i:984	s1:i:5847	s2:i:5734	de:f:0.0131	rl:i:403
......

read id: cc8739aa-9335-4a77-8681-f13b0b411a80
fast5(length:75742)

fastq(length:6848)
AAGTATTGCTTCGTTCA......GCAATACGTAACTTCGC

SEQ in bam file(length:6848)
GCGAAGTTACGTATTGC......TGAACGAAGCAATACTT(reverse complement of fastq, because the flag is 16)

read_references(length:6821)
TGTAAAATATCGAGTTCGCACATTT......CAACCCGCGCCAATTTATATCTA

read.flag :  16
read.query_name :  cc8739aa-9335-4a77-8681-f13b0b411a80
read.query_length :  6848
read.query_sequence :   GCGAAGTTACGTATTGCTTAGATATAAAT......GCAATACGTAACTGAACGAAGCAATACTT
read.query_alignment_length :  6800
read.query_alignment_start :  18
read.query_alignment_end :  6818
read.query_alignment_sequence :  TAGATATAAATTGGCGCGGGTTGTTT......ATGTGCGAACTCGATATTTTACA
read.reference_name :  ecoli_SCS110_chromosome
read.reference_start :  2803509
read.reference_end :  2810330
read.reference_length :  6821
read.get_reference_positions :  [2803509, 2803510, 2803511, 2803512, 2803513, ....  2810324, 2810325, 2810326, 2810327, 2810328, 2810329]

9f26aeb3-483b-44f8-b64c-37298959eb76	0	ENST00000351111.6	14	27	10S10M1D12M1D5M1D31M1D13M3D2M1I3M1I5M4D4M1D3M1I41M1I11M1D13M1D2M1I2M1D5M3D12M4D9M4I8M1D6M1I4M5D7M1D4M2D4M1D33M3I18M2D7M1D6M3I9M4D39M1D13M3D2M2D6M4D5M2I2M1D4M2I14M1I4M2I13M1D16M2I16M1D35M2D15M1D5M2D13M2D2M2D1M3D8M1D11M2I15M1D8M1D1M2D19M1D6M2D15M1I3M1I8M1D7M1D32M1D30M2I11M2D14M2D10M1D2M1D21M1D6M2D8M2D16M3I4M1D3M2I6M2D3M1I10M1D20M1I1M3I16M2I13M1I27M3I11M1I8M1I4M1D4M1D6M1I3M2I5M2I8M1D2M1D16M1I5M3I2M1D5M1D19M1I12M1I18M2D6M1D1M1D4M2D17M1D5M1I3M1I6M1D11M3I4M5D24M2D9M2D2M1D13M2I17M2D3M1I7M1D2M1D11M3D2M2I6M1D3M2D7M2I5M1D20M1I1M1I8M1I5M1D13M1D2M1I4M1D7M1D8M1D6M1D30M5D6M3D7M1D29M2I20M1D2M2D24M3D18M2D4M1D7M4S	*	0	0	CTGAAGACGTCGGCCGTGGCGGGACGCTGCGAGGGGTTCCTTGCCCAGGCGGGCCGGCTGCCTACCCTCAGACTGTCCGCTGCTTCCAAAGGCAAACCCATCCGTTGCGTGACGCAACCACAGCAGCAGAAGCTGATGGCTGCTGACTGAATATCCCCCCCGAAACCGCACACCACCCATGCCTAACGTTACCACCCCTGTTCCCACCGGGGAGATAAGGCCAGGCTTTCCGGGGATTGGCAGCAGTTCACCCTGACAACCGAATGATAATCGCCCACCGCCAGAATGTGTTAGGGTAAGAGGCTCACAAGCTCCGCGACACCAGCTGCGGAAACACAAGAACCAGACGAAGGTTCCCCCAACATGGTGTCCCTTGAGGAACTTCAAGTCTACCAAAATTTGCTCGCCCTACACTCTGCGAAGCCAACATGCTGCTGGTCGGAATGAAGAGCCCAAGGCAAGGAGATGGTACGGATCTTAAGGGTAATGCCATCTGCCGCTGTTAGGTGTTGCTGATGACACCATCCAATGGGCGGTTCAACAACTCCATAAGCTCCCCGGCCCGCCCTGGTGAGGGAGCTTGTGGGAGGCCTTCCTGCCACAGCCCAGACCCATCCCACCTGCACCGGACCAGCCCTCTAATTGTTTTCCCTGTAAAGTCAGTACTAAGAGAGCAACGCGAGAAGGATTCTGTCAACCGTCGGCTAATGAAGCCAGATCCTGACTGAGCCAGCTGCAGCCAGCCTGTTTAGCCAGCCTGCGTAAATACACTGCGTTATTGGCTGTATACCTCCCTTATAATGGCTTCGTGGAAGAACTGGGGTCGAGGAGTGTGTTTCGATTTCACTTGGTTTTACCTGCAGTAGTGATATTGTTCAGGTAATGGGCCACTTTGAGATGCAGCGGAGGATTCCATATTCAGATGCTCAGGACCAATTCGTTCCTATTAGTTCTTTCCCTATTGGACGTGATAGGAGCATAAGTCATTTCCCATTACCAGGTCCACCGAAGAGAGACTTGAAAAGATAAGGGCTATTGTCCCCCGCCCTTAGCTTGCCATGCTGCACGGGCTCCGGGCCTCTCTCCCCTTGGGCACAACCTATCGCCAACAGTAGCTCAACCAAGCAGTTGTTGAGAATGGCCAGCGAGAGCCTGCTGTGTGTGCCAGGCTTCGTTGAGGTGTTGTCAATATTAGTTCCACATTGCTGACACTGTACCCCTATTACCACAGAAGGAGCAGAGAAATATGGAGTGGCTCTGCTCAGGTCATGCAGTTGTAAAGTGTAGAACGGGACTTGATCAAGCCTCTGCACTGAAGACCGCGTCCTGAATTTCTAGAGCCTTATCGATTACCCGGAAGTAGGTCCCATCCACCACCGCCAGGTGTAATTTGATGCTGTTCCACCCTCGAAGCCGCTGCTGTGAGTTTGGAAGCACTGCTTCAATAAATGAATATC	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	NM:i:366	ms:i:898	AS:i:898	nn:i:0	ts:A:+	tp:A:P	cm:i:16	s1:i:157	s2:i:153	de:f:0.1832

read id: 9f26aeb3-483b-44f8-b64c-37298959eb76
fast5(length:58961)

fastq(length:1457)
CUGAAGACGUCGGCCGUGGCGGGACGCUG......UUUGGAAGCACUGCUUCAAUAAAUGAAUAUC

SEQ in bam file(length:1457)
CTGAAGACGTCGGCCGTGGCGGGACGCTG......TTTGGAAGCACTGCTTCAATAAATGAATATC

read.flag :  0
read.query_name :  9f26aeb3-483b-44f8-b64c-37298959eb76
read.query_length :  1457
read.query_sequence :  CTGAAGACGTCGGCCGTGGCGGGACGCTGC......TTTGGAAGCACTGCTTCAATAAATGAATATC
read.query_alignment_length :  1443
read.query_alignment_start :  10
read.query_alignment_end :  1453
read.query_alignment_sequence :  CGGCCGTGGCGGGACGCTGCGAGGGGTTCCTTGCCCA......AGTTTGGAAGCACTGCTTCAATAAATGAA
read.reference_name :  ENST00000351111.6
read.reference_start :  13
read.reference_end :  1527
read.reference_length :  1514