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computeMatrix with errors Mean of empty slice #1154

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Suger0917 opened this issue Aug 10, 2022 · 0 comments
Open

computeMatrix with errors Mean of empty slice #1154

Suger0917 opened this issue Aug 10, 2022 · 0 comments

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@Suger0917
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Dear deepTools group,

Thanks for your wonderful tool.
I used deeptools 3.5.1 and Python 3.9.12.

When I was running computeMatrix for ATAC-seq peaks using code:
computeMatrix reference-point
-S ESC_ATAC.bw
-R mm10_genecodeVM23.bed
--referencePoint TSS
-a 1000 -b 1000
-out matrix_Genes_ES_bulk.tab.gz
--outFileSortedRegions ES_bulk_region_order.bed

I got errors with thousands Skipping lines:
Skipping ENSMUST00000178569.1, due to being absent in the computeMatrix output.
/software/miniconda3/lib/python3.9/site-packages/deeptools/heatmapper.py:1219: RuntimeWarning:

Mean of empty slice

After that, results of plotHeatmap was empty.

Could you please give me some advice?
Thank you so much!

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