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I made an error when using metawrap to binning #527

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iMay1314 opened this issue Nov 28, 2023 · 1 comment
Open

I made an error when using metawrap to binning #527

iMay1314 opened this issue Nov 28, 2023 · 1 comment

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@iMay1314
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my code
metawrap binning -o bin_out -t 24 -m 200 -a all_contig/all_merge.fasta --metabat2 --maxbin2 --concoct all_fastq/*fastq
Error reported as follows
This is displayed in the terminal.
image

sorting the SRR10492802 alignment file
[bam_sort_core] merging from 24 files and 24 in-memory blocks...
[E::sam_hdr_sanitise] Malformed SAM header at line 2
samtools sort: failed to read header from "bin_out/work_files/tmp-samtools.0000.bam"
somthing  went wrong with sortin the alignments
Exiging

The header of sam file.
image
Request answers for me how to solve the problem. Thank you very much.
@Sautumn-Lin
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I also met this question. Have you solved?

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@iMay1314 @Sautumn-Lin