- A simple and convenient program to convert fasta sequences to fastq sequences, in casava 1.8 header format version.
- The fastq sequence quality scores will be ≥ 20 and distributed throughout the fastq sequence length, reference - https://support.illumina.com/help/BaseSpace_OLH_009008/Content/Source/Informatics/BS/QualityScoreEncoding_swBS.htm
where
@ - fastq header initial
LSM - unique instrument name
213 - run id
VGKE2RP - flowcell id
2 - flowcell lane
1971 - tile number within the flowcell lane
12668 - 'x'-coordinate of the cluster within the tile
04830 - 'y'-coordinate of the cluster within the tile
2 - member of a pair, 1 or 2 (paired-end or mate-pair reads only)
N - Y if the read is filtered, N otherwise
66 - 0 when none of the control bits are on, otherwise it is an even number
CGGACGTA - index 1 sequence
+ - connection between indexes
GTTCACGT - index 2 sequence
chmod +x fastA2Q
fastA2Q -i /path/input_file.fasta -o /path/output_file.fastq