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line 8: insilico_read_normalization.pl: command not found #55
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I have the exact same error. Any updates? |
Are u using conda? if the script was not found is because trinity cannot be located. The insilico normalization step is done using trinity scripts. cheers Sergio |
Thanks for the prompt response. Yes, using conda. Do I have to install trinity separately then?
|
Not really... the pipeline runs but breaks at normalization, correct? Sergio |
Yes, that is correct. |
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I'm getting the following error message while running the full analysis.
Error executing process > 'normalize_reads (control_R)'
Caused by:
Process
normalize_reads (control_R)
terminated with an error exit status (127)Command executed:
echo control_R
echo -e "\n-- Starting Normalization --\n"
mem=$( echo 150 GB | cut -f 1 -d " " )
insilico_read_normalization.pl --seqType fq -JM ${mem}G --max_cov 100 --min_cov 1 --left left-control_R.filter.fq --right right-control_R.filter.fq --pairs_together --PARALLEL_STATS --CPU 15
echo -e "\n-- DONE with Normalization --\n"
cat .command.out | grep "stats_file" -A 3 | tail -n 3 >control_R_normStats.txt
cp left.norm.fq left-"control_R".norm.fq
cp right.norm.fq right-"control_R".norm.fq
rm$(readlink -e left.norm.fq) $ (readlink -e right.norm.fq ) left.norm.fq right.norm.fq
Command exit status:
127
Command output:
control_R
-- Starting Normalization --
Command error:
control_R
-- Starting Normalization --
.command.sh: line 8: insilico_read_normalization.pl: command not found
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