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error in xpore diffmod #219
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Hi Laur (tagging you here @lyj95618),
Thanks! Best wishes, |
Thank you for your reply and the suggestion in another thread about changing the 9mer to 5mer! I have one more question about the xPore comparison analysis. Since I am combining data from R9 flowcell and R10(rna004) flowcell, is there a way xPore can adjust for the potential batch effect? My comparison condition: Sample A (R9), Sample B (R9), Sample1 (rna004) Vs Sample D (R9), Sample E (R9), Sample2 (rna004) Thanks, |
Hi,
I got the following error when I ran
xpore diffmod
This is my yaml for input
sample1 and sample2 were from R10 flowcell and the rest were from R9 flowcell. Since nanopolish doesn't support R10 data, I used f5c, which supports R10 and R9 and does the same thing as nanopolish, to process all the data. I noticed there are some differences in the eventalign.txt output. In the eventalign output file, the two samples from R10 have the 9 k-mer and the rest R9 data has 5 k-mer.
R10 data eventalign output:
R9 data:
Would this be the issue of why xpore outputs this error?
Thanks for the help!
Laur
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