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problem with syntax of command line #933

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mocherry opened this issue May 29, 2024 · 0 comments
Open

problem with syntax of command line #933

mocherry opened this issue May 29, 2024 · 0 comments

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@mocherry
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Dear all,

I am trying to analyze direct nanopore RNA sequencing data with salmon.
Now I seem to have problems with getting the command line properly:

I use:
salmon quant -i salmon_index -l r dmso_sham_4.fastq --validateMappings -o transcripts_quant

and get the following
Version Server Response: Not Found

salmon (selective-alignment-based) v1.10.3

[ program ] => salmon

[ command ] => quant

[ index ] => { salmon_index }

[ libType ] => { r }

[ ] => { dmso_sham_4.fastq }

[ validateMappings ] => { }

[ output ] => { transcripts_quant }

Logs will be written to transcripts_quant/logs
[2024-05-29 11:13:30.128] [jointLog] [info] setting maxHashResizeThreads to 24
Exception : [unknown library format string : R]
salmon quant was invoked improperly.
For usage information, try salmon quant --help
Exiting.
[2024-05-29 11:13:30.128] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
[2024-05-29 11:13:30.128] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
[2024-05-29 11:13:30.128] [jointLog] [info] Setting consensusSlack to selective-alignment default of 0.35.
[2024-05-29 11:13:30.128] [jointLog] [info] parsing read library format

So, as far I can see, being a bloody beginner, the error: Exception : [unknown library format string : R] somehow suggests that the small letter r in my command line is interpreted as a capital letter???
Or is there anything else wrong with my command?

Help would be appreciated. Thanks and best,
Matthias

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