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I am trying to analyze direct nanopore RNA sequencing data with salmon.
Now I seem to have problems with getting the command line properly:
I use:
salmon quant -i salmon_index -l r dmso_sham_4.fastq --validateMappings -o transcripts_quant
and get the following
Version Server Response: Not Found
salmon (selective-alignment-based) v1.10.3
[ program ] => salmon
[ command ] => quant
[ index ] => { salmon_index }
[ libType ] => { r }
[ ] => { dmso_sham_4.fastq }
[ validateMappings ] => { }
[ output ] => { transcripts_quant }
Logs will be written to transcripts_quant/logs
[2024-05-29 11:13:30.128] [jointLog] [info] setting maxHashResizeThreads to 24
Exception : [unknown library format string : R]
salmon quant was invoked improperly.
For usage information, try salmon quant --help
Exiting.
[2024-05-29 11:13:30.128] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
[2024-05-29 11:13:30.128] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
[2024-05-29 11:13:30.128] [jointLog] [info] Setting consensusSlack to selective-alignment default of 0.35.
[2024-05-29 11:13:30.128] [jointLog] [info] parsing read library format
So, as far I can see, being a bloody beginner, the error: Exception : [unknown library format string : R] somehow suggests that the small letter r in my command line is interpreted as a capital letter???
Or is there anything else wrong with my command?
Help would be appreciated. Thanks and best,
Matthias
The text was updated successfully, but these errors were encountered:
Dear all,
I am trying to analyze direct nanopore RNA sequencing data with salmon.
Now I seem to have problems with getting the command line properly:
I use:
salmon quant -i salmon_index -l r dmso_sham_4.fastq --validateMappings -o transcripts_quant
and get the following
Version Server Response: Not Found
salmon (selective-alignment-based) v1.10.3
[ program ] => salmon
[ command ] => quant
[ index ] => { salmon_index }
[ libType ] => { r }
[ ] => { dmso_sham_4.fastq }
[ validateMappings ] => { }
[ output ] => { transcripts_quant }
Logs will be written to transcripts_quant/logs
[2024-05-29 11:13:30.128] [jointLog] [info] setting maxHashResizeThreads to 24
Exception : [unknown library format string : R]
salmon quant was invoked improperly.
For usage information, try salmon quant --help
Exiting.
[2024-05-29 11:13:30.128] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
[2024-05-29 11:13:30.128] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
[2024-05-29 11:13:30.128] [jointLog] [info] Setting consensusSlack to selective-alignment default of 0.35.
[2024-05-29 11:13:30.128] [jointLog] [info] parsing read library format
So, as far I can see, being a bloody beginner, the error: Exception : [unknown library format string : R] somehow suggests that the small letter r in my command line is interpreted as a capital letter???
Or is there anything else wrong with my command?
Help would be appreciated. Thanks and best,
Matthias
The text was updated successfully, but these errors were encountered: