Home literacy environment (HLE) refers to the physical, interpersonal, and emotional / motivational factors in the home that have been found to be important for children’s literacy development. In this paper, the emergence of HLE research, its conceptualizations, and the effects of HLE factors, will be reviewed with an emphasis on the relations between HLE and children’s early literacy skills. Challenges faced by HLE researchers are also considered, and three aspects of issues are identified: privacy sensitivity, measure validity, and intervention fidelity. Apart from what is already known, this paper will also provide a summary of possible goals for future research.

The field of additive manufacturing, especially 3D printing, has gained growing attention in the research and commercial sectors in recent years. Notwithstanding that the capabilities of 3D printing have moved on to enhanced resolution, higher deposition rate, and a wide variety of materials, the crucial challenge of verifying that the component manufactured is within the dimensional tolerance as designed continues to exist. Material extrusion 3D printing has long been established for rapid prototyping and functional testing in many research and industry fields. However, its inconsistency and intrinsic defects (surface roughness and geometric inaccuracies) hinder its application in several areas, most notably “certify-as-you- build” small-batch prototyping and large-batch production.

In this study, we present an approach to reduce both inconsistency and the 3D geometric inaccuracies of products fabricated by material extrusion.

1. This work developed and demonstrated an approach for layer-by-layer mapping of 3D printed parts, which can be used for validation of printed models and in situ adjustment of print parameters. This in situ metrology system scans each layer at the time of printing, providing a 3D model of the as-printed part. A high-speed optical scanning system was integrated with a Material Extrusion type 3D printer to achieve in situ monitoring of dimensional inaccuracies during printing, which leaves the door open to implement a closed-loop feedback system to compensate geometric errors during printing in the future and fabricate “certify-as-you-build” products.

2. This work trained machine learning algorithms with data from this scanning system and predicted 3D geometric inaccuracies in new designs. Eight Conditional Adversarial Networks (CAN) machine learning models were trained on a limited number of scanned profile images of different layers, consisting of less than 50 actual images and 50 generated images, to predict the 3D geometric deviations of freeform shapes. The generated images were produced by randomly combining and cropping the actual images without any distortion. These CAN models produced predictions where at least 44.4%, 87.6%, 99.2% of data were within �0.05 mm, �0.10 mm, �0.15 mm of the actual measured value, respectively.

3. This work developed an Iterative Forward approach to redesign the Computer-Aided- Design model by reverse engineering using the trained machine learning models, allowing for compensation of print imperfection at the design stage, in advance of the first printing. The compensation algorithms with eight different sets of different parameters were evaluated. It has been proven that the Iterative Forward approach improved the geometric deviation of the predicted profiles by making compensation to the CAD model.

The cellular chaperone protein Hsc70, along with components of the 26S proteasome and ubiquitin-conjugated proteins have been shown to be sequestered in discrete foci in the nuclei of herpes simplex virus 1 (HSV-1) infected cells. We recently reported that cellular RNA polymerase II (RNAP II) undergoes proteasomal degradation during robust HSV-1 transcription, and that the immediate early protein ICP27 interacts with the C-terminal domain and is involved in the recruitment of RNAP II to viral transcription/replication compartments.

Here we show that ICP27 also interacts with Hsc70, and is required for the formation of Hsc70 nuclear foci. During infection with ICP27 mutants that are unable to recruit RNAP II to viral replication sites, viral transcript levels were greatly reduced, viral replication compartments were poorly formed and Hsc70 focus formation was curtailed. Further, a dominant negative Hsc70 mutant that cannot hydrolyze ATP, interfered with RNAP II degradation during HSV-1 infection, and an increase in ubiquitinated forms of RNAP II was observed. There was also a decrease in virus yields, indicating that proteasomal degradation of stalled RNAP II complexes during robust HSV-1 transcription and replication benefits viral gene expression.

We propose that one function of the Hsc70 nuclear foci may be to serve to facilitate the process of clearing stalled RNAP II complexes from viral genomes during times of highly active transcription.