Notes from Meeting 1/21/20

[gwaybio]

• Data from merged batches 5, 6, and 7 looking good so far
  • Batch correction not necessary
  • There might be some minor well effects
    • We discussed randomizing platemaps to the extent possible
• Morphology signature is interesting, and worth having a biologist dig a bit deeper into
  • Still some concern over confluence issues
    • While it doesn't seem to impact profiles, it is impacting explanatory morphology features that are typically only important in high confluence settings
• There might be something additionally interesting about the way the clones were selected
  • We talked about how they were all selected from the same cluster of resistant clones
    • Can you check me on this @mekelley? It is likely I didn't truly appreciate this point and I think it is important. (if possible, please add this to a new issue similar to Cloning Details #45 )
    • We know which cluster each sample belongs to which will help with:
      • Sequence specific clusters to determine molecular resistance mechanism
      • Use cluster info in future covariate models to observe which features are important for cluster of origin
• The Rockefeller team is actively onboarding new clones and a new proteasome inhibitor
  • They will present at next check-in
• We also briefly discussed presentations at next CSHL
  • It will be analysis heavy and @AnneCarpenter will represent (I don't remember if we've discussed this elsewhere before!)

[gwaybio]

The analysis we discussed is provided in #48