Beta-Lactamase DataBase (BLDB): Structure and Function

Kinetic parameters (work in progress)

Ambler Class Enzyme (Mutations) PubMedID

Benzylpenicillin
(BPN)

Penicillin G
(PCG)

Amoxicillin
(AMO)

Ampicillin
(AMP)

Oxacillin
(OXA)

Cloxacillin
(CLX)

Carbenicillin
(CRB)

Methicillin
(MTL)

Ticarcillin
(TIC)

Temocillin
(TEM)

Piperacillin
(PIP)

Azlocillin
(AZL)

Mezlocillin
(MZL)

Cefalothin
(CFT)

Cefuroxime
(CFU)

Cefotaxime
(CFX)

Ceftazidime
(CFZ)

Cefepime
(CFP)

Cefpirome
(CFR)

Cefoxitin
(CFN)

Cephaloridin
(CFD)

Cefazolin
(CEF)

Cefazolin (V0)
(CF0)

Cefazolin (Vss)
(CFS)

Cefsulodin
(CFI)

Aztreonam
(AZT)

Imipenem
(IMI)

Meropenem
(MER)

Ertapenem
(ERT)

Panipenem
(PAN)

Faropenem
(FAR)

Doripenem
(DOR)

Nitrocefin
(NIT)

Clavulanic acid
(CLA)

CENTA
(CEN)

A • GES-1 22908160 Show profile:	30 120 250		13 180 72						7 1000 7	0.002 4000 0.00005	8 900 9			900 5300 170		370 9000 40	110 40000 27.5	25 30000 0.8		0.08 60 1.3	540 4600 120					4.8 4000 1.2	0.007 1.5 5	0.0007 0.1 7	0.003 0.4 7.5				380 340 1100
A • GES-5 22908160 Show profile:	110 430 255		13 50 260						5 1600 3	0.003 4500 0.00007	160 1000 160			900 9000 100		36 25000 1.4	7 50000 0.14	8 90000 0.09		4.5 860 5	725 5000 145					0.06 1800 0.03	0.7 4.6 150	0.06 1 60	0.09 1.5 60				260 550 470
A • GES-11 22908160 Show profile:	4 37 110		7 37 190						2.6 37 70	0.015 550 0.03	27 120 255			740 4000 185		1600 5500 290	990 38000 26	9 930 10		0.02 13 1.5	455 3600 130					53 3200 17	0.015 1 15	0.0035 0.1 35	0.002 0.2 10				320 530 600
A • GES-12 22908160 Show profile:	16 60 270		16 73 220						4.6 214 21	0.16 1000 0.16	96 300 320			80 5300 15		586 9800 60	264 5350 50	8 1300 6		0.03 10 3	75 640 120					25 740 34	0.02 1 20	0.008 0.06 130	0.007 0.07 100				72 215 330
A • GES-14 22908160 Show profile:	23 300 77		7 130 54						0.2 32 6	0.02 400 0.05	60 750 80			1100 6000 180		14 7900 1.8	2.5 20000 0.125	0.4 600 0.7		0.35 220 1.6	150 4400 34					3.5 5000 0.7	0.09 2.9 31	0.01 0.2 50	0.01 0.3 33				56 135 415
A • GES-18 23114760 Show profile:	70 400 175		7 40 175						5 1000 5	0.004 3500 0.001	75 700 100			500 6000 80		>20 >20000 1	>2.5 >20000 0.125	>1 >20000 0.05		3.4 1000 3	360 4600 70					0.08 3000 0.02	0.4 3.5 110	0.04 1 40	0.06 1.3 40				100 330 300
A • KPC-2 18625772 Show profile:	45 44 1000			20 29 700					ND NH 0		40 51 800			106 141 750	66 160 410	66 174 380	ND NH 0		12 251 500							3 35 900	20 88 230
A • KPC-2 20662006 Show profile:											51 15 3300			44 14 3100		142 207 700											19 19 1000					0.73 5.5 130	52 6 8500
A • KPC-2 22687511 Show profile:											65 12 5400			223 38 5900		140 190 700											21 21 1000						130 8 16000
A • KPC-2 W105F 20662006 Show profile:											112 24 4700			219 27 8100		102 200 500											9 15 600						96 11 8700
A • KPC-2 W105N 20662006 Show profile:											89 34 2600			219 47 4700		ND >200 0											3.1 33 90						104 24 4300
A • KPC-2 W105L 20662006 Show profile:											ND >100 0			175 177 1000		ND >200 0											1.5 24 60					0.75 4.8 210	97 61 1600
A • KPC-2 W105V 20662006 Show profile:											50 37 1400			94 127 1500		ND >200 0											5.5 83 70						103 62 1700
A • KPC-2 T237S 20421396 Show profile:											42 6 7000			65 18 3600		43 168 260											21 21 1000						50 5 10000
A • KPC-2 T237A 20421396 Show profile:											42 12 3500			26 30 900		<0.1 ND ND											6.8 31 220						191 15 13000
A • KPC-2 R220H 22687511 Show profile:											68 13 5200			206 108 1900		ND ND 0											9 11 800						260 16 16000
A • KPC-2 R220A 22687511 Show profile:											193 164 1200			379 207 1800		ND ND 0											4 18 200						327 54 6100
A • KPC-2 R220M 22687511 Show profile:											85 35 2400			156 98 1600		ND ND 0											10 28 400						724 94 7700
A • KPC-2 R220K 22687511 Show profile:											40 23 1700			88 45 2000		ND ND 0											7 5 1400						120 10 12000
A • KPC-2 E276A 22687511 Show profile:											59 12 4800			157 24 6500		118 278 400											11 12 900						97 4.9 19000
A • KPC-2 E276D 22687511 Show profile:											38 5.2 7200			139 12 1100		100 253 500											13 10 1300						77 4.1 19000
B1 • IMP-1 19770275 Show profile:		320 520 620		950 200 4800							ND ND 0			48 21 2400	8 37 220	1.3 4 350	8 44 180	7 11 660		16 8 2000						>0.01 >1000 0	46 39 1200	50 10 120
B1 • NDM-1 19770275 Show profile:		11 16 680		15 22 660							14 12 1170			4 10 400	5 8 610	6 10 580	5 181 30	13 77 170		1 49 20						ND ND 0	20 94 210	12 49 250						ND ND 0
B1 • VIM-2 19770275 Show profile:		56 49 1140									33 72 450			57 44 1280	12 22 550	28 32 860	89 98 900	5 184 30		3 24 120						<0.5 ND 0	10 10 990	1 5 280
B3 • BJP-1 20696874 Show profile:				670 19					ND 0		700 67			230 580			>700 4.3										260 60	190 830
B3 • FEZ-1 20696874 Show profile:				>5000 11					>5000 13		4200 12			120 2500			>1000 4										>1000 200	85 500
B3 • GOB-1 20696874 Show profile:									150 520		170 1700			24 670			71 760										60 660	5 5300
B3 • L1 20696874 Show profile:				40 4400					140 2700		20 7000			8.9 9200			145 200										90 730	10 4500
D • OXA-1 20086146 Show profile:				357 64 6000	577 31 19000																16 80 210												94 9 10000
D • OXA-2 16870757 Show profile:	90 4 22000			110 25 4400	900 360 2500									3.4 7 490			0.02 12 1.7
D • OXA-20 9687410 Show profile:		26 4.4 5900		80 33 2400	116 329 350	70 788 88			4 147 30					13 5 2600			ND ND 0			ND ND 0	20 69 300					6 69 90
D • OXA-22 10898703 Show profile:	0.1 <2 >40		0.1 6 16		ND ND 0	0.7 5 140			0.1 9 10		ND ND 0			0.9 3 330	0.05 5 10	ND ND 0	ND ND 0			0.05 10 4	5.5 10 550					ND ND 0
D • OXA-29 11709332 Show profile:		65 10 6500		107 16 6700	34 4.4 7700		13 16 810	27 41 660			31 39 790				>0.19 >250 0.79	0.17 128 1.3	>0.24 >500 0.49					11 30 370				0.21 210 1	NH NH 0	NH NH 0					96 1800 19000
D • OXA-32 11796380 Show profile:	3.5 45 80		1.5 220 70			2.5 110 20			1 60 15		3 155 20			3 60 50		ND ND 0	H >3000 H		ND ND 0		2 360 6					ND ND 0
D • OXA-40 12499201 Show profile:	5 23 220			5 220 20	2 876 3				1 60 20		1 23 50			3 72 50		ND NH 0	20 2500 10	ND NH 0	ND NH 0		5 1000 5					ND NH 0	0.1 6.5 15	ND NH 0
D • OXA-46 15855521 Show profile:		18 48 380		6 20 300	300 320 940		85 ; 5 730 ; 545 1200 ; 9.2			NH >1000 0		11 46 240	12 30 400	8 23 350	NH >1000 0	NH >1000 0	NH >1000 0	NH >1000 0					17 23 740	7 40 170	NH >1000 0	NH >1000 0							20 18 1100
D • OXA-48 23305656 Show profile:	245 40 6100			340 5200 65	25 30 850				45 55 820	0.3 45 6.5	75 410 180			3 20 150		10 190 60	4 5100 1	1 160 6								ND NH 0	2 14 145	0.1 200 0.5	NA NA NA
D • OXA-48 19477418 Show profile:				955 395 2400	130 95 1400					0.3 45 6.6				44 195 230		>9 >900 10	ND NH 0	>0.6 >550 1.1		>0.05 >200 0.26							4.8 13 370	0.07 11 6.2	0.13 100 1.3	1.4 14 100	0.038 13 2.9		940 120 7700
D • OXA-54 14693565 Show profile:	120 60 2000			540 4300 125	35 75 500				30 240 125		20 240 85			3 230 15		15 1600 10	ND NH 0	1 180 5	4 110 35		1 45 20					ND NH 0	1 4 250	0.1 125 1
D • OXA-57 15793160 Show profile:		0.4 29.5 13.6		0.54 71.9 7.54	1.23 40.7 30.2	0.51 18.7 17.9					2.11 21.3 99			0.28 163 1.73							13 97.9 133												63 17.9 3510
D • OXA-57 D170N 15793160 Show profile:		0.61 56.3 10.7		2.12 38.9 54.4	2.37 37.2 63.7	1.12 166 6.69					5.7 22.1 258			0.86 100 8.59																			79.5 50.4 1580
D • OXA-57 K232N 15793160 Show profile:		0.75 46.6 16		1.42 157 8.98	3.07 194 15.7	1.04 180 5.77					4.31 9.48 455			0.65 55.4 11.7																			29.7 13.2 2240
D • OXA-85b 16870757 Show profile:	830 10 83000			45 76 570	780 36 21000				>10 >250 40					4.1 47 87	>1.8 >400 4.5	ND NH 0	ND NH 0	ND NH 0																	0.42 23 18
D • OXA-114a 18362192 Show profile:	0.1 10 10				<0.01 ND 0				0.1 40 2.5		0.5 1 100			0.5 15 30		<0.01 ND 0	<0.01 ND 0			<0.01 ND 0							>0.1 >2000 0.05
D • OXA-163 21422200 Show profile:	15 40 380			25 320 80					1 320 3		8 70 110			3 80 40		30 850 35	200 >2000 1000	2 350 6								0.5 230 2	0.03 530 0.05	0.07 2200 0.03	0.01 150 0.1
D • OXA-181 23305656 Show profile:	445 125 3560			200 130 1500	70 80 875				65 100 650	0.2 85 2.5	75 210 350			3 125 25		4 75 55	ND NH 0	15 1120 15								ND NH 0	4 40 100	0.1 70 1	0.1 85 1
D • OXA-232 23305656 Show profile:	510 60 8500			380 175 2200	240 100 2400				480 135 3600	0.04 70 0.5	380 540 700			7 125 50		6 195 30	ND NH 0	13 1200 10								ND NH 0	1 35 30	0.1 125 1	0.1 100 1

NOTE: In each cell, the first value is k_cat (s^-1), the second value is Km (µM) and the third value is k_cat/Km (s^-1·mM^-1). When two values are present on the same line, the first correspond to V₀ and the second to V_ss.

Abbreviations: ND - not determinable; NH – no hydrolysis detected; NA – not available.

Statistics (number of kinetics): Overall (47); class A (21); subclass B1 (3); subclass B2 (0); subclass B3 (4); class C (0); class D (19).

Last updated: November 20, 2024.

If you use BLDB please cite: Naas, T.; Oueslati, S.; Bonnin, R. A.; Dabos, M. L.; Zavala, A.; Dortet, L.; Retailleau, P.; Iorga, B. I., Beta-Lactamase DataBase (BLDB) – Structure and Function. J. Enzyme Inhib. Med. Chem. 2017, 32, 917-919.

The development of the BLDB database was funded in part by the JPIAMR transnational project DesInMBL, the Région Ile-de-France (DIM Malinf) and the Laboratory of Excellence in Research on Medication and Innovative Therapeutics (LERMIT).

Contact: [email protected]

Live statistics (since December 3rd, 2023)